A Sensitive Fluorescent Assay for Trypsin Activity in Biological Samples using BSA-Au Nanoclusters

نویسندگان

  • Xianxiang Wang
  • Yanying Wang
  • Hanbing Rao
  • Zhi Shan
چکیده

Neste artigo, foi desenvolvido um novo método fluorométrico, simples, sensível e seletivo, para a medição de tripsina em amostras biológicas. O método foi baseado na medição da supressão da intensidade da fluorescência de agregados de nanopartículas de ouro estabilizados com albumina sérica bovina (BSA), pela proteólise enzimática. A curva de calibração para tripsina foi atingida no intervalo de concentração de 1-60 nmol L com coeficiente de correlação 0,995 e um limite de detecção de 0,6 nmol L. O método foi também usado satisfatoriamente para avaliação da atividade da tripsina e os resultados mostraram que os valores das constantes de Michaelis-Menten (Km) e catalítica (Kcat), de tripsina para estes substratos de nanoagreagados BSA-Au, foram 1,6×10 -5

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تاریخ انتشار 2012